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In this work, a rapid detection method for studying the viability of L.pneumophila cells combining Propidium Monoazide (PMA) treatment and qPCR hasbeen developed. This compound specifically intercalates and cleaves the genomic DNAfrom dead cells that cannot be amplified by polymerasechain reaction (PCR). This method,applied to water samples spiked with L. pneumophila, has demonstrated a signalreduction of 4.74log if the results are compared with those obtained by qPCR withoutPMA, and similar results if compared with the culture isolation method. Thiscombined assay has also proved to be useful for specific detection of cultivable L.pneumophila cells from environmental samples. Nevertheless, the qPCR combined withPMA underestimate the number of viable cells by approximately 0.6 log compared toculture isolation counts. Includes 9 references, tables, figures.

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Edition: Vol. - No. Published: 11/01/2009 Number of Pages: 8File Size: 1 file , 810 KB