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This extended abstract presents a two year AWWARF-funded study to investigate the relativeperformance of all methods approved for use for the detection of coliforms and E. coliin drinking water in the U.S. and United Kingdom. This study investigated both the sensitivity andselectivity of each method. Many of the methods performed similarly, but those thatutilize lactose fermentation for the detection of coliforms performed significantlyworse than those that are based upon the detection of expression -D-galactosidase.The recovery of coliform organisms was significantly lower with methods usinglactose-based media using naturally contaminated samples. The numbers of coliformsdetected by methods using -D-galactosidase as the detection system was higher dueto the number of slow lactose fermenting organisms present. However, while therewere some differences in the genera detected, the differences were not significant.Methods utilizing -D-galactosidase detected more of the "common coliforms" suchas Klebsiella, Citrobacter and Enterobacter than did lactose based methods as well asmore recently described coliforms. Includes extended abstract only.

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Edition: Vol. - No. Published: 11/01/2009 Number of Pages: 2File Size: 1 file , 770 KB