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Several real-time quantitative PCR methods, including direct PCR and RT-PCRmethods, and methods based on PCR and RT-PCR integrated with tissue cell culture(ICC-PCR and ICC-RT-PCR) were used to evaluate ultraviolet (UV) inactivation of adenovirus 41(Ad 41). Ad 41 (TAK strain) grown on HEK293 cells were exposed to UV doses of 40,80, 160 and 320 mJ/cmsup2/sup using a collimated beam apparatus. When compared to thetraditional cytopathic effects (CPE) based cell culture infectivity assay, direct real-timequantitative PCR and RT-PCR assays (i.e. without cell culture) were found to beunreliable for measuring the level inactivation of Ad41 by UV. Viruses which lost theirinfectivity were still detected by the direct molecular methods. UV inactivation of Ad41measured by ICC-RT-PCR, on the other hand, was not statistically different thaninactivation measured by the CPE assay. UV inactivation of Ad41 measured by the ICCPCRassay was slightly lower than inactivation measured by CPE and ICC-RT-PCR.Therefore, a cell culture assay based on viral quantification using real-time quantitativeRT-PCR was found to be a potentially attractive substitute for the CPE-based cell cultureinfectivity assay for assessing virus inactivation by UV. Includes 11 references, table, figures.

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Edition: Vol. - No. Published: 11/01/2008 Number of Pages: 7File Size: 1 file , 770 KB