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Adenoviruses are a diverse group of viruses which infect a variety of host animals. There are51 serotypes which can infect humans, causing eye and respiratory infections, potentiallysevere enteric dysentery, infections of the urinary tract, and occasionally even problems in thecentral nervous system. Adenovirus has recently become a focus of the water treatmentcommunity because of its emerging role as a significant human pathogen and its apparentresistance to ultraviolet (UV) disinfection. Several papers have been published on UV inactivation ofadenovirus and other viruses using monochromatic low pressure UV (LP UV) followed byassays of infectivity using cell culture (Gerba et al., 2002; Meng and Gerba, 1996; Thurston-Enriquez et al., 2003). Cell culture infectivity assays require using a host cell system whichdoes not allow direct assessment of the virus itself. Polymerase chain reaction (PCR)technologies for investigation of DNA have also been applied to studies of adenovirus; however,these studies have involved either combinations of PCR and cell culture for tests of viralinfectivity after UV treatment or simple detection of adenoviral DNA in untreated environmentalsamples (Choi and Jiang, 2005; Ko et al., 2005). This study examines the application of aquantitative PCR assay to directly assess damage to the adenoviral genome after UVirradiation. Methods described include: Propagation of Host Cells; Adenovirus Preparation and Enumeration; UV Irradiation; MPN plating; and, DNA Extraction and PCR. Includes 6 references, figures.

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Edition: Vol. - No. Published: 11/01/2007 Number of Pages: 3File Size: 1 file , 130 KB