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AWWA WQTC65998

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AWWA WQTC65998 Characterization of Amino Acid Fraction of Organic Matter during Drinking Water Treatment

Conference Proceeding by American Water Works Association, 11/01/2007

Westerhoff, Paul; Dotson, Aaron

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Amino acids and amino sugars (AAAS) are important for drinking water treatment,because they can serve as substrate for biological activity, can serve as indicators for solublemicrobial products, and may be precursors for the formation of nitrogenous disinfection byproducts.Despite these important needs, very little work is available to understand the nature ofamino acids in drinking waters. The goal of this project was to measure amino acids in dissolvedorganic matter (DOM) isolates and bulk waters from drinking water sources.The samples, both bulk water and DOM, were collected from waters affected by seasonalalgal blooms or wastewater discharges. Bulk water samples were collected from 10 full scalewater treatment plants (WTPs) across the country during the summer/fall of 2006. The sampleswere analyzed for a multitude of water quality including: dissolved organic carbon (DOC),ultraviolet absorbance (UVA), total dissolved nitrogen (TDN), dissolved inorganic nitrogen(DIN), and dissolved organic nitrogen (DON). A 500 milliliter (mL) aliquot was concentratedand freeze dried for determination of AAAS.DOM was fractionated and isolated from two wastewater discharges, a drinking waterreservoir, and two laboratory cultures of algae and bacteria. DOM was fractionated according toLeenheer et. al (2007), which enriched selected fractions with organic nitrogen. The isolateswere characterized by the following methods: Fourier transform infrared spectroscopy (FTIR); solid-state nuclearmagnetic resonance (NMR); and, elemental analysis. The isolated DOM fractions were freezedried and stored in a desiccator. For this study, the nitrogen rich colloidal organic matterfraction is discussed. Includes 8 references, tables, figure.

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Edition: Vol. - No. Published: 11/01/2007 Number of Pages: 4File Size: 1 file , 84 KB