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AWWA JAW64356

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AWWA JAW64356 Journal AWWA - Laser Scanning Cytometry for Enumeration of Fluorescent Microspheres

Journal Article by American Water Works Association, 03/01/2007

Ladner, David A.; Lee, Benjamin W.; Clark, Mark M.

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As surrogates for viruses, bacteria, and protozoa,fluorescent microspheres can be used to challengepathogen-removal technologies and determine theirefficacy in removing contaminants of interest. In addition,microspheres are helpful in quantifying flow patternsand mixing characteristics. However, the mostcommon microsphere enumeration method, directcount by fluorescence microscopy, requires skilledoperators and expensive equipment. In the researchdiscussed here, a prototype device that used laser scanningcytometry and that was constructed of relativelyinexpensive components performed at least as well asthe microscope for the range of ~5-200 S/mmsup2/sup.Among the many challenges facing water providerstoday are increasingly stringent regulations and thethreat of intentional contamination of water sourceswith pathogens. A device such as the prototypedescribed here would offer utility managers a way totest their water supply technologies with more accuracyand precision. It represents a building block towardmore advanced (but still low-cost) technologies thatcan detect pathogens and help reduce risks. Includes 7 references, figures.

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Edition: Vol. 99 - No. 3 Published: 03/01/2007 Number of Pages: 8File Size: 1 file , 620 KB